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《医学前沿（英文）》 2017年 第11卷 第2期 页码 247-252 doi: 10.1007/s11684-017-0519-6
The activity of free radicals in follicular fluid was related to ovarian responsiveness, in vitro fertilization (IVF), and embryo transfer success rate. However, studies analyzing the relationship between the free radical scavenging capacity and embryo quality of infertile women with polycystic ovarian syndrome (PCOS) were lacking. The aim of this study was to evaluate the relationship between the free radical scavenging window of women with PCOS and their embryo quality. The free radical scavenging capacity of follicular fluid from women with PCOS was determined by a,a-diphenyl-b-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) assay, superoxide radical, and reactive oxygen species (ROS) assay. In the DPPH and ROS assays, the follicular fluid from grades I and II embryos was significantly higher than the follicular fluid from grades III and IV embryos. The lower control limit of DPPH radical scavenging capacity and upper control limit of ROS level were 13.2% and 109.0 cps, respectively. The calculated lower control limit and upper control limit were further confirmed in the follicular fluid of embryos of all grades. These cut-off values of free radical scavenging activity of follicular fluid could assist embryologists in choosing the development of embryos in PCOS patients undergoing IVF.
关键词： in vitro fertilization PCOS free radical embryo quality
Mara Simopoulou, Konstantinos Sfakianoudis, Petroula Tsioulou, Anna Rapani, Polina Giannelou, Nikolaos Kiriakopoulos, Agni Pantou, Nikolaos Vlahos, George Anifandis, Stamatis Bolaris, Konstantinos Pantos, Michael Koutsilieris
《医学前沿（英文）》 2019年 第13卷 第6期 页码 627-638 doi: 10.1007/s11684-019-0697-5
关键词： artificial ovary artificial uterus artificial gametes assisted reproduction considerations in vitro fertilization
Optimum nitrogen fertilization of
Wentao ZOU,Ruifeng JIA,Jinchang YANG,Rongsheng LI,Guangtian YIN
《农业科学与工程前沿（英文）》 2016年 第3卷 第4期 页码 368-374 doi: 10.15302/J-FASE-2016120
关键词： Calophyllum inophyllum growth nitrogen fertilization nutrient status photosynthesis
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《医学前沿（英文）》 2016年 第10卷 第4期 页码 517-521 doi: 10.1007/s11684-016-0480-9
This study aimed to compare the antimicrobial activities of topical agents against Propionibacterium acnes isolated from patients admitted to a hospital in Shanghai, China. The minimal inhibitory concentrations of the cultured P. acnes were determined in accordance with the Clinical and Laboratory Standards Institute. Susceptibilities to clindamycin and erythromycin were compared in terms of gender, age, disease duration, previous treatment, and disease severity. A total of 69 P. acnes strains were isolated from 98 patients (70.41%). The susceptibility to triple antibiotic ointment (neomycin/bacitracin/polymyxin B) and bacitracin was 100%. The susceptibility to fusidic acid was 92.7%. The resistance rates to neomycin sulfate, erythromycin, and clindamycin were 11.7%, 49.3%, and 33.4%, respectively. The high resistance rate to clindamycin and erythromycin was significantly affected by gender, previous treatment, and disease severity rather than by age and disease duration. Topical antibiotics should not be used separately for long-term therapy to avoid multiresistance. The use of topical antibiotics should be determined by clinicians on the basis of clinical conditions.
关键词： antimicrobial susceptibility/resistance Propionibacterium acnes topical antibiotics in vitro study
Kaifeng RAO, Na LI, Mei MA, Zijian WANG
《环境科学与工程前沿（英文）》 2014年 第8卷 第1期 页码 69-78 doi: 10.1007/s11783-013-0502-7
关键词： waste water in vitro recombinant yeast assay ethoxyresorufin-O-deethylase (EROD) receptor endocrine
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《医学前沿（英文）》 2016年 第10卷 第1期 页码 41-51 doi: 10.1007/s11684-016-0429-z
Midline2 (MID2) is an ubiquitin-conjugating E2 enzyme linked to tumor progression and a novel interacting partner of breast cancer 1, early-onset (BRCA1). However, the role of MID2 in breast cancer remains unknown. This study investigated the expression, prognostic value, and role of MID2 in breast cancer. The expression of MID2 mRNA and protein was significantly upregulated in breast cancer tissue and established cell lines compared with that in normal breast epithelial cells and paired adjacent non-tumor tissue (P<0.001). Immunohistochemical analysis demonstrated that MID2 was overexpressed in 272 of 284 (95.8%) paraffin-embedded, archived breast cancer tissue. Moreover, MID2 expression increased with advanced clinical stage (P<0.001). High MID2 expression was significantly associated with advanced clinical stages and T, N, and M staging (all P<0.05). Univariate and multivariate analyses indicated that high MID2 expression was an independent prognostic factor for poor overall survival in the entire cohort (93.73 vs. 172.1 months; P<0.001, log-rank test) and in subgroups with stages Tis+ I+ II and III+ IV. Furthermore, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide colony formation, and anchorage-independent growth ability assays were conducted. Results showed that siRNA silencing of MID2 expression significantly reduced MCF-7 and MDA-MB-231 cell proliferation in vitro and blocked the growth of MDA-MB-231 cell xenograft tumors in vivo (P<0.05). This study indicated that MID2 may be a novel prognostic marker and interventional target in breast cancer.
关键词： breast cancer MID2 proliferation overall survival xenograft
IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI
《农业科学与工程前沿（英文）》 2021年 第8卷 第4期 页码 635-644 doi: 10.15302/J-FASE -2020343
The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of Eucalyptus tereticornis, Xanthium sibiricum, Artemisia argyi, Tupistra chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plant pathogenic fungi Aspergillus niger, Botrytis cinerea, Penicillium digitatum, P. expansum, P. italicumand Rhizopus stolonifer. All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v), 95% ethanol (v/v), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. T. chinensis may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.
关键词： Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis
INTERACTIVE KNOWLEDGE LEARNING BY ARTIFICIAL INTELLIGENCE FOR SMALLHOLDERS
《农业科学与工程前沿（英文）》 2023年 第10卷 第4期 页码 648-653 doi: 10.15302/J-FASE-2023505
Enhancement of farming management relies heavily on enhancing farmer knowledge. In the past, both the direct learning approach and the personnel extension system for improving fertilization practices of smallholders has proven insufficiently effective. Therefore, this article proposes an interactive knowledge learning approach using artificial intelligence as a promising alternative. The system consists of two parts. The first is a dialog interface that accepts information from farmers about their current farming practices. The second part is an intelligent decision system, which categorizes the information provided by farmers in two categories. The first consists of on-farm constraints, such as fertilizer resources, split application times and seasons. The second comprises knowledge-based practices by farmers, such as nutrient in- and output balance, ratios of different nutrients and the ratios of each split nutrient amount to the total nutrient input. The interactive knowledge learning approach aims to identify and rectify incorrect practices in the knowledge-based category while considering the farmer’s available finance, labor, and fertilizer resources. Investigations show that the interactive knowledge learning approach can make a strong contribution to prevention of the overuse of nitrogen and phosphorus fertilizers, and mitigating agricultural non-point source pollution.
关键词： artificial intelligence extension system non-point source pollution control smallholders fertilization
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《医学前沿（英文）》 2018年 第12卷 第3期 页码 307-318 doi: 10.1007/s11684-017-0547-2
Acute ischemic stroke (AIS), as the third leading cause of death worldwide, is characterized by its high incidence, mortality rate, high incurred disability rate, and frequent reoccurrence. The neuroprotective effects of extract (GBE) against several cerebral diseases have been reported in previous studies, but the underlying mechanisms of action are still unclear. Using a novel rat cortical capillary endothelial cell-astrocyte-neuron network model, we investigated the neuroprotective effects of GBE and one of its important constituents, Ginkgolide B (GB), against oxygen–glucose deprivation/reoxygenation and glucose (OGD/R) injury. In this model, rat cortical capillary endothelial cells, astrocytes, and neurons were cocultured so that they could be synchronously observed in the same system. Pretreatment with GBE or GB increased the neuron cell viability, ameliorated cell injury, and inhibited the cell apoptotic rate through Bax and Bcl-2 expression regulation after OGD/R injury. Furthermore, GBE or GB pretreatment enhanced the transendothelial electrical resistance of capillary endothelial monolayers, reduced the endothelial permeability coefficients for sodium fluorescein (Na-F), and increased the expression levels of tight junction proteins, namely, ZO-1 and occludin, in endothelial cells. Results demonstrated the preventive effects of GBE on neuronal cell death and enhancement of the function of brain capillary endothelial monolayers after OGD/R injury ; thus, GBE could be used as an effective neuroprotective agent for AIS/reperfusion, with GB as one of its significant constituents.
关键词： acute ischemic stroke Ginkgo bilobaextract Ginkgolide B network model neuroprotection
《农业科学与工程前沿（英文）》 2022年 第9卷 第2期 页码 284-294 doi: 10.15302/J-FASE-2021435
Bioenergy, currently the largest renewable energy source in the EU (64% of the total renewable energy consumption), has sparked great interest to meet the 32% renewable resources for the 2030 bioeconomy goal. The design of innovative cropping systems informed by bioeconomy imperatives requires the evaluate of the effects of introducing crops for bioenergy into conventional crop rotations. This study aimed to assess the impacts of changes in conventional cropping systems in mixed dairy cattle farms redesigned to introduce bioenergy crops either by increasing the biomass production through an increase of cover crops, while keeping main feed/food crops, or by substituting food crops with an increase of the crop rotation length. The assessment is based on the comparison between conventional and innovative systems oriented to feed and biogas production, with and without tillage, to evaluate their agri-environmental performances (biomass production, nitrogen fertilization autonomy, greenhouse gas emissions and biogas production). The result showed higher values in the biogas cropping system than in the conventional and feed ones for all indicators, biomass productivity (27% and 20% higher, respectively), nitrogen fertilization autonomy (26% and 73% higher, respectively), methanogenic potential (77% and 41% higher, respectively) and greenhouse gas emissions (15% and 3% higher, respectively). There were no negative impacts of no-till compared to the tillage practice, for all tested variables. The biogas cropping system showed a better potential in terms of agri-environmental performance, although its greenhouse gas emissions were higher. Consequently, it would be appropriate to undertake a multicriteria assessment integrating agri-environmental, economic and social performances.
《农业科学与工程前沿（英文）》 2022年 第9卷 第3期 页码 396-406 doi: 10.15302/J-FASE-2022456
● Interflow acts as the dominant pathway for N loss loadings.
关键词： improved fertilization regime interflow nitrogen forms nitrogen leaching purple soil sloping cropland
Hong GAO,Jie SONG,Shibin SHANG,Zhanqian SONG
《农业科学与工程前沿（英文）》 2017年 第4卷 第1期 页码 106-115 doi: 10.15302/J-FASE-2017133
关键词： dehydroabietic acid-based diarylamine DFT study fluorescent probe in vitro imaging
Biodegradable polymethacrylic acid grafted psyllium for controlled drug delivery systems
Ranvijay KUMAR, Kaushlendra SHARMA
《化学科学与工程前沿（英文）》 2013年 第7卷 第1期 页码 116-122 doi: 10.1007/s11705-013-1310-0
关键词： psyllium acetyl salicylic acid in-vitro drug release swelling biodegradation
John NSOR-ATINDANA, Maoshen CHEN, Liu WEI, Khin Myat NOE, Yue LI, Fang ZHONG
《农业科学与工程前沿（英文）》 2018年 第5卷 第3期 页码 340-350 doi: 10.15302/J-FASE-2018209
Interest in understanding the digestion behavior of starch in the presence of dietary fibers is growing due to the ability of dietary component to control the release and absorption of glucose. This presents an outstanding opportunity to improve the quality of food products by incorporating dietary fiber into starchy food products. The physicochemical properties of different fibers and their behavior in the gastrointestinal tract (GIT) differ. To test the efficacy of these different fibers on starch digestion, static in vitro digestion models under conditions that mimic the human GIT are frequently used. Indeed, many efforts have been committed to the development of various static in vitro protocols for starch digestion. Though not considered as the gold standard in digestibility studies in food science and technology, static simulated models provide a useful alternative to in vivo techniques for rapid screening of the digestibility of food products under conditions that simulate the human GIT. This review presents the current status and development of digestion techniques for simulating digestion conditions in the human GIT, with particular interest on starch digestion in the presence of dietary fiber in the three phases of digestions including the oral, gastric and the intestinal steps. This summary can benefit investigators in developing static in vitro digestion models designed to simulate starch digestion with relevant values of the quantifiable parameters, including pH, enzymes and simulated digestive fluids.
关键词： dietary fiber in vitro digestion nutrition simulation starch
谢兰,刘冉,冯娟,邢婉丽,程京
《中国工程科学》 2017年 第19卷 第2期 页码 29-36 doi: 10.15302/J-SSCAE-2017.02.005
分子诊断是个体化医学的重要组成部分，已成为体外诊断行业的主要增长动力。近年来，尽管我国的分子诊断市场规模较小，但增长迅速。本研究通过文献研究、专家研讨、实地考察等方式，对我国的分子诊断行业进行市场分析，研究我国分子诊断行业的现状及特点，理清该产业在创新、产业转化及相关政策法规上面临的机遇和挑战，提出克服现有管理体制制约的对策建议，对推动产业健康发展具有重要的意义。
标题 作者 时间 类型 操作
Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality
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期刊论文
What will the future hold for artificial organs in the service of assisted reproduction: prospects and considerations
Mara Simopoulou, Konstantinos Sfakianoudis, Petroula Tsioulou, Anna Rapani, Polina Giannelou, Nikolaos Kiriakopoulos, Agni Pantou, Nikolaos Vlahos, George Anifandis, Stamatis Bolaris, Konstantinos Pantos, Michael Koutsilieris
期刊论文
Optimum nitrogen fertilization of
Wentao ZOU,Ruifeng JIA,Jinchang YANG,Rongsheng LI,Guangtian YIN
期刊论文
Antimicrobial activity of topical agents against Propionibacterium acnes: an in vitro study of clinical
null
期刊论文
In vitro agonistic and antagonistic endocrine disrupting effects of organic extracts from waste water
Kaifeng RAO, Na LI, Mei MA, Zijian WANG
期刊论文
and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation in vitro
null
期刊论文
extract and Ginkgolide B against oxygen–glucose deprivation/reoxygenation and glucose injury in a new in vitro
null
期刊论文
AGRI-ENVIRONMENTAL ASSESSMENT OF CONVENTIONAL AND ALTERNATIVE BIOENERGY CROPPING SYSTEMS PROMOTING BIOMASS PRODUCTIVITY
期刊论文
EFFECTS OF SUBSTITUTION OF MINERAL NITROGEN WITH ORGANIC AMENDMENTS ON NITROGEN LOSS FROM SLOPING CROPLAND OF PURPLE SOIL
期刊论文
Biodegradable polymethacrylic acid grafted psyllium for controlled drug delivery systems
Ranvijay KUMAR, Kaushlendra SHARMA
期刊论文
Implications of static
John NSOR-ATINDANA, Maoshen CHEN, Liu WEI, Khin Myat NOE, Yue LI, Fang ZHONG
期刊论文
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